rabbit anti psd 93  (Alomone Labs)

Journal: PLoS ONE

Article Title: Phosphoprotein Associated with Glycosphingolipid-Enriched Microdomains Differentially Modulates Src Kinase Activity in Brain Maturation

doi: 10.1371/journal.pone.0023978

Figure Lengend Snippet: A ) Whole brain lysates from WT mice of the indicated ages were Western blotted and stained for PSD93. B ) Brains of P1 wild type and Pag1 -/- mice and 3 month old WT/ Pag1 -/- littermate pairs were lysed at concentrations of 3 ml/g and 4.5 ml/g, respectively, in GEM-lysis buffer and subjected to sucrose-density centrifugation. Fractions were analyzed by Western blotting and staining for PSD93. Lipid raft fractions were identified by binding of cholera toxin subunit B (Ctx) binding to GM1-lipids in a dot blot from the fractions. C ) Densitometric quantification of the PSD93 signal in GEM-fractions as percentage of the total PSD93-signal. For both time points, the PSD93 signal in fractions 2-5 was expressed as a ratio to control. This most likely overestimates the lipid raft localized material in P1, as only fraction 2 and 3 contain rafts (n = 3 independent experiments for each time point) D ) Csk-immunoprecipitates from whole brain lysates of WT mice (ages as indicated), stained for PSD93 and Csk. Densitometric quantification of the relative amount of PSD93 bound to Csk in brains of P1 and 3 month old mice (n = 3 independent experiments) E ) Csk-immunoprecipitates from whole brain lysates of 3 month old WT and Pag1 -/- mice stained for PSD93 and Csk. Two exposures are given for the PSD93-signal in the left panel. Comparison of whole brain lysates of 3 month old WT and Pag1 -/- to P1. Given are the means +/- SEM, n.s. not significant, Student's t-test.

Article Snippet: Rabbit phosphor-specific anti-Src (pY418), recognizing both Y423 in mouse Src and Y419 in mouse Fyn, as well as anti-Src (pY529), recognizing both Y534 in mouse Src and Y530 in mouse Fyn due to the highly conserved sequence of these residues were purchased from BioSource, rabbit anti-Csk (C-20) and anti-Ctk from Santa Cruz, mouse-anti-Csk (clone 52) and mouse anti-CHK from BD BioSciences, mouse anti-Src (clone GD11) from Upstate (Lake Placid, NY), rabbit anti-PSD93 from Alomone Labs Ltd., rabbit anti-c-Src (Y215) from ECM Biosciences, and mouse anti-β-actin (clone AC-15) from Sigma.

Techniques: Western Blot, Staining, Lysis, Centrifugation, Binding Assay, Dot Blot

Journal: Scientific Reports

Article Title: An altered glial phenotype in the NL3 R451C mouse model of autism

doi: 10.1038/s41598-020-71171-y

Figure Lengend Snippet: PSD-95 protein levels are increased in the striatum of NL3 R451C mice, whereas PSD-93 and NL2 expression is unchanged. Levels of postsynaptic proteins, PSD-95, PSD-93 and NL2 in cortical ( A , G , M ), striatal ( C , I , O ) and cerebellar ( E , K , Q ) brain lysates from NL3 R451C and WT mice were analysed via Western blot. Densitometric analysis was performed to demonstrate quantitative expression of PSD-95 ( B , D , F ), PSD-93 ( H , J , L ) and NL2 ( N , P , R ) relative to β actin expression. Genotype differences were analysed using an unpaired, two-tailed Student’s t-test (n = 6 mice in each group); *p < 0.05. Data represented as mean ± SEM.

Article Snippet: Rabbit polyclonal anti-PSD-93 (110 kDa) , Alomone Labs #APZ002 , 1:500.

Techniques: Expressing, Western Blot, Two Tailed Test

Journal: Scientific Reports

Article Title: An altered glial phenotype in the NL3 R451C mouse model of autism

doi: 10.1038/s41598-020-71171-y

Figure Lengend Snippet: Antibodies used in Western blot analysis.

Article Snippet: Rabbit polyclonal anti-PSD-93 (110 kDa) , Alomone Labs #APZ002 , 1:500.

Techniques: Western Blot